RNA

Part:BBa_K4410000

Designed by: Ruyi Shi   Group: iGEM22_Worldshaper-HZBIOX   (2022-09-19)


fragment H1 from E.coli Nissle 1917

This part is an up-stream sequence of argR gene from the genome of E. coli Nissle 1917. The argR gene knockout method is to use the λ-Red homologous recombination system, so it is necessary to add argR homology arms upstream and downstream of the replacement gene for subsequent gene knockout. This part is the upstream argR homology arm.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 305
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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